APC (phospho Ser2054) rabbit pAb

• Background:

  This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]

• Description:

  This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008],

• UniProt:

  P25054

• Swiss Prot:

  P25054

• Reactivity:

  Human; Rat; Mouse

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human APC around the phosphorylation site of Ser2054. AA range:2020-2069

• Clonality:

  Polyclonal

• Source:

  Rabbit

• Applications:

  WB; IHC; IF; ELISA

• Concentration:

  1 mg/ml

• Dilution:

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

• Molecular Weight:

  311kD

• Storage Conditions:

  -20°C/1 year

• Observed Molecular Weight:

  311kD

• Fragment:

  IgG

• Subcellular Location:

  Cell junction, adherens junction . Cytoplasm, cytoskeleton . Cell projection, lamellipodium . Cell projection, ruffle membrane . Cytoplasm . Cell membrane . Associated with the microtubule network at the growing distal tip of microtubules (PubMed:19632184) . Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment (PubMed:19151759) . The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane (PubMed:20937854) . .

• Other Product Names:

  APC; DP2.5; Adenomatous polyposis coli protein; Protein APC; Deleted in polyposis 2.5

• Gene ID (Human):

  324