ERK 8 (phospho Thr175/Y177) rabbit pAb

ERK 8 (phospho Thr175/Y177) rabbit pAb

• Description: Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Domain: The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteosomal degradation. Domain: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. enzyme regulation: Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1. function: In vitro, phosphorylates MBP. PTM: Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro. PTM: Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase. similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. similarity: Contains 1 protein kinase domain. subunit: Interacts with CSK/c-Src, ABL1, RET and TGFB1I1. tissue specificity: Widely expressed with a maximal expression in lung and kidney.
• Synonyms: MAPK15; ERK7; ERK8; Mitogen-activated protein kinase 15; MAP kinase 15; MAPK 15; Extracellular signal-regulated kinase 7; ERK-7; Extracellular signal-regulated kinase 8; ERK-8
• Gene ID: 225689
• UniProt: Q8TD08
• Cellular Locus: Cytoplasm, cytoskeleton, cilium basal body. Cell junction, tight junction. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasmic vesicle, autophagosome. Golgi apparatus. Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Co-localizes to the cytoplasm only in presence of ESRRA (PubMed:21190936). Translocates to the nucleus upon activation (PubMed:20638370). At prometaphase I, metaphase I (MI), anaphase I, telophase I, and metaphase II (MII) stages, is stably detected at the spindle (By similarity)..
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Immunogen: The antiserum was produced against synthesized peptide derived from human ERK8 around the phosphorylation site of Thr175 and Tyr177. AA range:141-190
• Clonality: Polyclonal
• Validated Applications: WB, IHC, IF, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
• Molecular Weight: 60kD
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.