CENP-A (phospho Ser7) rabbit pAb

CENP-A (phospho Ser7) rabbit pAb

• Description: Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015],
• Synonyms: CENPA; Histone H3-like centromeric protein A; Centromere autoantigen A; Centromere protein A; CENP-A
• Gene ID: 1058
• UniProt: P49450
• Cellular Locus: Nucleus. Chromosome, centromere, kinetochore. Chromosome, centromere. Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height (PubMed:19114591). Phosphorylation at Ser-68 during early mitosis abolishes association with chromatin and centromeres and results in dispersed nuclear location (PubMed:25556658)..
• Host: Rabbit
• Species Reactivity: Human, Rat, Mouse,
• Immunogen: The antiserum was produced against synthesized peptide derived from human Centromeric Protein A around the phosphorylation site of Ser7. AA range:1-50
• Clonality: Polyclonal
• Validated Applications: IF, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.