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Separase rabbit pAb

CAT: 0855-ES3423-02Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0855-ES3423-02Size:100 µL
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Background
Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]) .[supplied by OMIM, Nov 2010]
Description
Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al. 2009 [PubMed 19345191]). [supplied by OMIM, Nov 2010],
Product Name Alternative
ESPL1; ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase
UniProt
Q14674
Swiss Prot
Q14674
Reactivity
Human; Mouse
Immunogen
The antiserum was produced against synthesized peptide derived from human SEPARASE. AA range:767-816
Target
Separase
Clonality
Polyclonal
Source
Rabbit
Applications
WB; IHC; IF; ELISA
Concentration
1 mg/ml
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Buffer
-20°C/1 year
Molecular Weight
230kD
Storage Conditions
-20°C/1 year
Observed Molecular Weight
230kD
Fragment
IgG
Subcellular Location
Cytoplasm. Nucleus.
Other Product Names
ESPL1; ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase
Gene ID (Human)
9700

UniProtKB · Q14674

Separin

ESPL1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q14674
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ESPL1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.4.22.49
Processing
—
Secondary accessions
—
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

PTM

Autocatalytic cleavagePhosphoprotein

Biological process

Chromosome partition

Cellular component

CytoplasmNucleus

Molecular function

HydrolaseProteaseThiol protease

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