PSK-H1 rabbit pAb

PSK-H1 rabbit pAb

• Description: Catalytic activity: ATP + a protein = ADP + a phosphoprotein. enzyme regulation: Activity depends on Ca (2+) concentration. function: May be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing. PTM: Autophosphorylated on serine residues. PTM: Myristoylated. Required for membrane association. Prerequisite for palmitoylation to occur. PTM: Palmitoylated. similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. similarity: Contains 1 protein kinase domain. subcellular location: Localized in the Brefeldin A-sensitive Golgi compartment, at centrosomes, in the nucleus with a somewhat speckle-like presence, membrane-associated to the endoplasmic reticulum (ER) and the plasma membrane (PM), and more diffusely in the cytoplasm. Found to concentrate in splicing factor compartments (SFCs) within the nucleus of interphase cells. The acylation-negative form may be only cytoplasmic and nuclear. Acylation seems to allow the sequestering to the intracellular membranes. Myristoylation may mediate targeting to the intracellular non-Golgi membranes and palmitoylation may mediate the targeting to the Golgi membranes. Dual acylation is required to stabilize the interaction with Golgi membranes. subunit: Homodimer. tissue specificity: Expressed in all tissues and cell lines tested with the highest level of abundance in testis.
• Synonyms: PSKH1; Serine/threonine-protein kinase H1; Protein serine kinase H1; PSK-H1
• Gene ID: 5681
• UniProt: P11801
• Cellular Locus: Golgi apparatus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus speckle. Endoplasmic reticulum membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Cytoplasm. Localized in the brefeldin A-sensitive Golgi compartment, at centrosomes, in the nucleus with a somewhat speckle-like presence, membrane-associated to the endoplasmic reticulum (ER) and the plasma membrane (PM), and more diffusely in the cytoplasm. Found to concentrate in splicing factor compartments (SFCs) within the nucleus of interphase cells. The acylation-negative form may be only cytoplasmic and nuclear. Acylation seems to allow the sequestering to the intracellular membranes. Myristoylation may mediate targeting to the intracellular non-Golgi membranes and palmitoylation may mediate the targeting t
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Immunogen: The antiserum was produced against synthesized peptide derived from human KPSH1. AA range:261-310
• Clonality: Polyclonal
• Validated Applications: WB, IHC, IF, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Molecular Weight: 48kD
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.