PCNA rabbit pAb

• Background :

  The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

• Description :

  The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008],

• UniProt :

  P12004

• Swiss Prot :

  P12004

• Reactivity :

  Human; Mouse; Rat; Monkey; Rabbit

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human PCNA. AA range:61-110

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; IHC; IF; ELISA

• Concentration :

  1 mg/ml

• Dilution :

  WB 1:500-2000; IHC-p 1:100-500; IF/ICC 1:100-500; ELISA 1:5000-20000

• Molecular Weight :

  30-33kd

• Storage Conditions :

  -20°C/1 year

• Observed Molecular Weight :

  30-33kd

• Fragment :

  IgG

• Subcellular Location :

  Nucleus . Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902) . Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase (PubMed:15543136) . Co-localizes with SMARCA5/SNF2H and BAZ1B/WSTF at replication foci during S phase (PubMed:15543136) . Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. .

• Other Product Names :

  PCNA; Proliferating cell nuclear antigen; PCNA; Cyclin

• Gene ID (Human) :

  5111