G3BP1 rabbit pAb

• Background:

  This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

• Description:

  This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008],

• UniProt:

  Q13283

• Swiss Prot:

  Q13283

• Reactivity:

  Human; Mouse; Monkey

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human G3BP-1. AA range:199-248

• Clonality:

  Polyclonal

• Source:

  Rabbit

• Applications:

  WB; IHC; IF; ELISA

• Concentration:

  1 mg/ml

• Dilution:

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

• Molecular Weight:

  60kD

• Storage Conditions:

  -20°C/1 year

• Observed Molecular Weight:

  60kD

• Fragment:

  IgG

• Subcellular Location:

  Cytoplasm, cytosol . Perikaryon . Cytoplasm, Stress granule . Nucleus . Cytoplasmic in proliferating cells (PubMed:11604510) . Cytosolic and partially nuclear in resting cells (PubMed:11604510) . Recruited to stress granules in response to arsenite treatment (PubMed:12642610, PubMed:20180778) . The unphosphorylated form is recruited to stress granules (PubMed:12642610) . HRAS signaling contributes to this process by regulating G3BP dephosphorylation (PubMed:12642610) . .

• Other Product Names:

  G3BP1; G3BP; Ras GTPase-activating protein-binding protein 1; G3BP-1; ATP-dependent DNA helicase VIII; hDH VIII; GAP SH3 domain-binding protein 1

• Gene ID (Human):

  10146