Dok-3 rabbit pAb

• Background:

  Domain:PTB domain mediates receptor interaction., function:DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL function., PTM:Constitutively tyrosine-phosphorylated., PTM:On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase., similarity:Belongs to the DOK family. Type A subfamily., similarity:Contains 1 IRS-type PTB domain., similarity:Contains 1 PH domain., subunit:On tyrosine phosphorylation, interacts with CSK and INPP5D/SHIP1 via their SH2 domains. Both Tyr-381 and Tyr-398 are required for interaction with INPP5D. Only Tyr-381 is required for interaction with CSK. Binds ABL through the PTB domain and in a kinase-dependent manner. Does not interact with RasGAP., tissue specificity:Expressed in spleen.

• Description:

  Domain: PTB domain mediates receptor interaction. function: DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL function. PTM: Constitutively tyrosine-phosphorylated. PTM: On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase. similarity: Belongs to the DOK family. Type A subfamily. similarity: Contains 1 IRS-type PTB domain. similarity: Contains 1 PH domain. subunit: On tyrosine phosphorylation, interacts with CSK and INPP5D/SHIP1 via their SH2 domains. Both Tyr-381 and Tyr-398 are required for interaction with INPP5D. Only Tyr-381 is required for interaction with CSK. Binds ABL through the PTB domain and in a kinase-dependent manner. Does not interact with RasGAP. tissue specificity: Expressed in spleen.

• UniProt:

  Q7L591

• Swiss Prot:

  Q7L591

• Reactivity:

  Human; Mouse

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human DOK3. AA range:101-150

• Clonality:

  Polyclonal

• Source:

  Rabbit

• Applications:

  WB; IHC; IF; ELISA

• Concentration:

  1 mg/ml

• Dilution:

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

• Molecular Weight:

  58kD

• Storage Conditions:

  -20°C/1 year

• Observed Molecular Weight:

  58kD

• Fragment:

  IgG

• Subcellular Location:

  Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side .

• Other Product Names:

  DOK3; Docking protein 3; Downstream of tyrosine kinase 3

• Gene ID (Human):

  79930