Cytochrome b5 rabbit pAb

• Background:

  The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

• Description:

  The protein encoded by this gene is a membrane-bound Cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010],

• Synonyms:

  CYB5A; CYB5; Cytochrome b5; Microsomal cytochrome b5 type A; MCB5

• Gene ID:

  1528

• UniProt:

  P00167

• Cellular Locus:

  [Isoform 1]: Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side. Microsome membrane; Single-pass membrane protein; Cytoplasmic side.; [Isoform 2]: Cytoplasm.

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Reactivity:

  Human; Mouse; Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human CYB5. AA range:61-110

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Source:

  Rabbit

• Applications:

  WB, IHC, IF, ELISA

• Validated Applications:

  WB, IHC, IF, ELISA

• Stability:

  -20°C/1 year

• Concentration:

  1 mg/mL

• Dilution:

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

• Molecular Weight:

  15kD

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  15 kD

• Subcellular Location:

  [Isoform 1]: Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side. Microsome membrane; Single-pass membrane protein; Cytoplasmic side.; [Isoform 2]: Cytoplasm.

• Other Product Names:

  CYB5A; CYB5; Cytochrome b5; Microsomal cytochrome b5 type A; MCB5

• Gene ID (Human):

  1528

• SwissProt (Human):

  P00167