YAP (phospho Ser127) rabbit pAb

YAP (phospho Ser127) rabbit pAb

• Description: This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013],
• Synonyms: YAP1; YAP65; Yorkie homolog; 65 kDa Yes-associated protein; YAP65
• Gene ID: 10413
• UniProt: P46937
• Cellular Locus: Cytoplasm. Nucleus. Both phosphorylation and cell density can regulate its subcellular localization (PubMed:18158288, PubMed:20048001). Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus (PubMed:18158288, PubMed:20048001). At low density, predominantly nuclear and is translocated to the cytoplasm at high density (PubMed:18158288, PubMed:20048001, PubMed:25849865). PTPN14 induces translocation from the nucleus to the cytoplasm (PubMed:22525271). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm at the blastocyst and epiblast stages (By similarity)..
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat, Monkey
• Immunogen: The antiserum was produced against synthesized peptide derived from human YAP around the phosphorylation site of Ser127. AA range:93-142
• Clonality: Polyclonal
• Validated Applications: IF, WB, IHC, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
• Molecular Weight: 65kD
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.