Rad17 (phospho Ser645) rabbit pAb
CAT:
855-ES1425-01
Size:
50 μL
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Rad17 (phospho Ser645) rabbit pAb
- Description: The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, h
- Synonyms: RAD17; R24L; Cell cycle checkpoint protein RAD17; hRad17; RF-C/activator 1 homolog
- Gene ID: 5884
- UniProt: O75943
- Cellular Locus: Nucleus. Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
- Host: Rabbit
- Species Reactivity: Human, Mouse
- Immunogen: The antiserum was produced against synthesized peptide derived from human RAD17 around the phosphorylation site of Ser645. AA range:621-670
- Clonality: Polyclonal
- Validated Applications: WB, ELISA, IHC
- Stability: 1 year
- Concentration: 1 mg/mL
- Dilution: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
- Molecular Weight: 77kD
- Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.