EZClick™ Myristoylated Protein Assay Kit, Red Fluorescence

EZClick™ Myristoylated Protein Assay Kit, Red Fluorescence

• Description: Myristoylation is a co- and post-translational modification of proteins. Myristoylated proteins are involved in cellular trafficking, cellular transformation and oncogenesis. In this modification, Myristic acid, a saturated fatty acid is covalently conjugated to a protein through the action of N-myristoyl transferase (NMT). NMT transfers the myristate moiety from Myristoyl-CoA to an N-terminal glycine of a target protein. The NMT enzyme is associated with carcinogenesis. The upregulation of its expression and activity is observed in colorectal cancer, gall-bladder cancer, brain tumor etc. BioVision offers EZClick™ Myristoylated Protein Assay Kit, a highly specific, simple and robust tool for labeling and detection of myristoylated proteins. In this assay, an Alkynyl Myristic Acid is added directly to the cells that get incorporated into proteins during or post translation followed by the click reaction with the azide-containing dye. This system offers a powerful method for imaging localization, trafficking and dynamics of Myristoylated proteins, or detection by FACS for quantitative studies. Labeled Myristoylated Proteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-azide or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format.
• Reactivity: Mammalian
• Applications: Detection and quantification of biosynthesis, dynamics and turnover of myristic acid modified proteins, Identification and localization of myristic acid modified proteins, Screening for genotoxic compounds and effectors of protein modifications
• Detection Method: FACS and Microscopy
• Shipping Conditions: Gel Pack
• Storage Conditions: -20°C
• Shelf Life: 12 months