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cGMP ELISA Kit

CAT: 0026-E4717-100Size: 96 AssaysDry Ice: NoHazardous: No
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CAT#:0026-E4717-100Size:96 Assays
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Description
cGMP ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cGMP. During the reaction, cGMP in the sample or standard competes with a fixed amount of cGMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to cGMP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of cGMP in the samples is then determined by comparing the OD of the samples to the standard curve.
CAS Number
7732-18-5
Reactivity
All
Applications
Specificity: This kit recognizes cGMP in samples. No significant cross-reactivity or interference between cAMP and analogues was observed., Detection Range: 0.78-50 pmol/ml, Repeatability: Coefficient of variation is < 10%., Sensitivity: 0.47 pmol/ml
Detection Method
Absorbance-450 nm
Shipping Conditions
Gel Pack
Storage Conditions
4°C
Shelf Life
12 months

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