CGMP ELISA KIT

• Background:

  Guanosine 3’, 5’-cyclic monophosphate (cGMP) is a key second messenger molecule in intracellular signal transduction. Monitoring cGMP levels is one of the most common ways to screen for agonists and antagonists of G protein–coupled receptors (GPCRs) . Bioyeargene cGMP ELISA Kit is based on the competition between HRP-labeled cGMP and free cGMP for fixed amounts of mouse monoclonal anti-cAMP antibody (cGMP MAb) binding sites. Because the concentration of the HRP-labeled cGMP is held constant while the concentration of cGMP varies, the amount of HRP-labeled cGMP that is able to bind to the cGMP MAb will be inversely proportional to the concentration of cGMP in the well. This antigen-antibody complex binds to the goat anti-mouse IgG that has been previously attached to the well. The plate is washed to remove any unbound reagents and then developed. The ELISA typically displays an IC50 (50% B/B0) of approximately 5 pmol/mL and a detection limit of approximately 0.1 pmol/mL.

• Specifications:

  CGMP

• Detection Method:

  Colorimetric

• Assay Type:

  Quantitative

• Sample Type:

  Plasma, Serum

• Detection Range:

  0.78-100 pmol/mL

• Recovery:

  80-120%

• Sensitivity:

  0.78 pmol/mL

• Shipping Conditions:

  2-8 °C

• Storage Conditions:

  The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition.

• Notes:

  For research use only.