Rabbit anti Rat G Protein b5-D1

• Background:

  The G beta5 protein is a G protein subunit present predominantly in the central nervous system. This protein appears to be expressed in two forms, one found in the brain (39 kDa) and one found in the retina (44 kDa). It has also been found that Gb5 associates tightly with RGS7, a member of the superfamily of negative regulators of G protein signaling. These complexes have been localized to most regions of the brain, whereas G 1-4 exhibit no observable colocalization with RGS7, despite their widespread codistribution throughout similar neuronal networks.

• UniProt:

  O14775

• Host:

  Rabbit

• Species Reactivity:

  Murine, Rat, Human

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Source:

  Rabbits were immunized with a unique peptide sequence derived from the D1 blade of the G 5 protein

• Applications:

  WB

• Field of Research:

  Signal Transduction

• Purification Method:

  Ammonium Sulfate Precipitation

• Assay Principle:

  Antibody can be used for Western blotting (5-10 µg/mL). Optimal concentration should be evaluated by serial dilutions.

• Stability:

  See expiration date on vial

• Concentration:

  See vial for Concentration

• Form:

  This antibody is specific for a peptide sequence within the D1 blade which is; Not found in the G betas 1-4._x000D_ Provided as solution in phosphate buffered saline with 0,08% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Liang, J.J., et al. 'RGS7 complex formation and colocalization with the Gbeta5 subunit in the adult rat and influence on Gbeta5gamma2-mediated PLCbeta signaling.' J. Neurosci. Res. 2000: 60, 58-64_x000D_ 2. Cabrera, J.L., et al. 'Identification of the Gbeta5-RGS7 protein complex in the retina.' Biochem Biophys Res Commun. 1998: 249, 898-902_x000D_ 3. Watson, A.J., et al. 'A novel form of the G protein beta subunit Gbeta5 is specifically expressed in the vertebrate retina.' J. Biol. Chem. 1996: 271, 28154-28160

• Shipping Conditions:

  Ambient Temperature, freeze upon arrival

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  WB

• CAS Number:

  9007-83-4