Anti-Hu TCR gamma/delta APC

Anti-Hu TCR gamma/delta APC

• Background: The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
• Specifications: The mouse monoclonal antibody 11F2 recognizes an extracellular epitope in all molecular forms of the TCR gamma/delta.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Purified CD3 antigen complex
• Target Antigen: TCR gamma/delta
• Isotype: IgG1
• Clone: 11F2
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: APC
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Ichinohasama R, Miura I, Takahashi T, Yaginuma Y, Myers J, DeCoteau JF, Yee C, Kadin ME, Mori S, Sawai T: Peripheral CD4+ CD8- gammadelta T cell lymphoma: a case report with multiparameter analyses. Hum Pathol. 1996 Dec27(12):1370-7., URL: https://pubmed.ncbi.nlm.nih.gov/8958314/,*Futagbi G, Welbeck JE, Tetteh JK, Hviid L, Akanmori BD: Selective activation of TCR-gammadelta+ cells in endemic Burkitt's lymphoma. Malar J. 2007 May 236:69., URL: https://pubmed.ncbi.nlm.nih.gov/17521425/,*Bertaina A, Zorzoli A, Petretto A, Barbarito G, Inglese E, Merli P, Lavarello C, Brescia LP, De Angelis B, Tripodi G, Moretta L, Locatelli F, Airoldi I: Zoledronic acid boosts γδ T-cell activity in children receiving αβ + T and CD19 + cell-depleted grafts from an HLA-haplo-identical donor. Oncoimmunology. 2016 Sep 276(2):e1216291., URL: https://pubmed.ncbi.nlm.nih.gov/28344861/,*Papadopoulou M, Tieppo P, McGovern N, Gosselin F, Chan JK, Goetgeluk G, Dauby N, Cogan A, Donner C, Ginhoux F, Vandekerckhove B, Vermijlen D: TCR sequencing reveals the distinct development of fetal and adult human Vγ9Vδ2 T cells. J Immunol. 2019 Sep 15203(6):1468-1479., URL: https://pubmed.ncbi.nlm.nih.gov/31413106/,*Borst J, van Dongen JJ, Bolhuis RL, Peters PJ, Hafler DA, de Vries E, van de Griend RJ: Distinct molecular forms of human T cell receptor gamma/delta detected on viable T cells by a monoclonal antibody. J Exp Med. 1988 May 1167(5):1625-44., URL: https://pubmed.ncbi.nlm.nih.gov/2966845/,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.