Anti-Hu CD62E Purified

• Description:

  CD62E (E-selectin) is a type I transmembrane glycoprotein of 97 - 115 kDa. It recognizes carbohydrate ligands on various molecules, and promotes leukocyte extravasation by mediating leukocyte rolling on activated endothelia during inflammation. CD62E is expressed above all in inflammated endothelium, but it can also be found on resting endothelium in certain organs, such as skin, or placenta.

• Certification:

  RUO

• Reactivity:

  Human

• Immunogen:

  TNF-activated HUVEC and human CD40 cDNA-transfected cells

• Target Antigen:

  CD62E

• Clone:

  HAE-1f

• Applications:

  FC (QC tested), ICC

• Concentration:

  1 mg/mL

• Format:

  Purified

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Tu L, Chen A, Delahunty MD, Moore KL, Watson SR, McEver RP, Tedder TF: L-selectin binds to P-selectin glycoprotein ligand-1 on leukocytes: interactions between the lectin, epidermal growth factor, and consensus repeat domains of the selectins determine ligand binding specificity. J Immunol. 1996 Nov 1;157 (9) :3995-4004., URL: https://pubmed.ncbi.nlm.nih.gov/8892633/, *Trappenburg MC, van Schilfgaarde M, Marchetti M, Spronk HM, ten Cate H, Leyte A, Terpstra WE, Falanga A: Elevated procoagulant microparticles expressing endothelial and platelet markers in essential thrombocythemia. Haematologica. 2009 Jul;94 (7) :911-8. , URL: https://www.ncbi.nlm.nih.gov/pubmed/19508974

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  The mouse monoclonal HAE-1f recognizes an extracellular epitope of CD62E, a transmembrane glycoprotein expressed on activated endothelial cells, as well as in skin, placenta, and bone marrow endothelium.

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-4 μg/ml.

• Isotype:

  Mouse IgG1 kappa