Anti-Hu CD8 Purified

• Description:

  The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.

• Certification:

  RUO

• Immunogen:

  Human blood lymphocytes

• Target Antigen:

  CD8

• Clone:

  LT8

• Applications:

  FC (QC tested), IP

• Concentration:

  1 mg/mL

• Format:

  Purified

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Ivanidze J, Hoffmann R, Lochmüller H, Engel AG, Hohlfeld R, Dornmair K: Inclusion body myositis: laser microdissection reveals differential up-regulation of IFN-γ signaling cascade in attacked versus nonattacked myofibers. Am J Pathol. 2011 Sep;179 (3) :1347-59., URL: https://pubmed.ncbi.nlm.nih.gov/21855683/, *Agea E, Russano A, Bistoni O, Mannucci R, Nicoletti I, Corazzi L, Postle AD, De Libero G, Porcelli SA, Spinozzi F: Human CD1-restricted T cell recognition of lipids from pollens. J Exp Med. 2005 Jul 18;202 (2) :295-308., URL: https://www.ncbi.nlm.nih.gov/pubmed/16009719, *Held K, Bhonsle-Deeng L, Siewert K, Sato W, Beltrán E, Schmidt S, Rühl G, Ng JK, Engerer P, Moser M, Klinkert WE, Babbe H, Misgeld T, Wekerle H, Laplaud DA, Hohlfeld R, Dornmair K: αβ T-cell receptors from multiple sclerosis brain lesions show MAIT cell-related features. Neurol Neuroimmunol Neuroinflamm. 2015 May 7;2 (4) :e107., URL: https://www.ncbi.nlm.nih.gov/pubmed/25977934 Ivani, *Nelson M, Loveday M: Exploring the innate immunological response of an alternative nonhuman primate model of infectious disease; the common marmoset. J Immunol Res. 2014;2014:913632., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=m+nelson+m+loveday Agea E Russano A Bistoni O Mannucci R Nicoletti I Corazzi L Postle AD De Libero G Porcelli SA Spinozzi F: Human CD1-restricted T cell recognition of lipids from pollens. J Exp Med. 2005 Jul

• Other References:

  *Devine L, Thakral D, Nag S, Dobbins J, Hodsdon ME, Kavathas PB: Mapping the binding site on CD8 beta for MHC class I reveals mutants with enhanced binding. J Immunol. 2006 Sep 15;177 (6) :3930-8., URL: https://pubmed.ncbi.nlm.nih.gov/16951356/, *Pang DJ, Hayday AC, Bijlmakers MJ: CD8 Raft localization is induced by its assembly into CD8alpha beta heterodimers, not CD8alpha alpha homodimers. J Biol Chem. 2007 May 4;282 (18) :13884-94., URL: https://pubmed.ncbi.nlm.nih.gov/17341584/, *van den Berg HA, Wooldridge L, Laugel B, Sewell AK: Coreceptor CD8-driven modulation of T cell antigen receptor specificity. J Theor Biol. 2007 Nov 21;249 (2) :395-408., URL: https://pubmed.ncbi.nlm.nih.gov/17869274/

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  The mouse monoclonal antibody LT8 recognizes an extracellular epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. This antibody blocks Leu2 binding.

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-4 μg/ml.

• Species:

  Non-human primates, Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1