High Sensitivity beta-Galactosidase Substrate (HSPG)

• Description:

  High Sensitivity beta-Galactosidase Substrate (HSPG)_x000D_ Catalog number: B2010525_x000D_ Lot number: Batch Dependent_x000D_ Expiration Date: Batch dependent_x000D_ Amount: 100 mg_x000D_ Molecular Weight or Concentration: N/A_x000D_ Supplied as: Powder_x000D_ Applications: substrate in the development of ultra-sensitive beta-galactosidase-based assays_x000D_ Storage: ˆ’20°C_x000D_ Keywords: b-gal substrate, CEDIA_x000D_ Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ References:_x000D_ 1: Itahana K, Campisi J, Dimri GP. Methods to detect biomarkers of cellular_x000D_ senescence: the senescence-associated beta-galactosidase assay. Methods Mol_x000D_ Biol. 2007;371:21-31._x000D_ _x000D_ 2: Smale ST. Beta-galactosidase assay. Cold Spring Harb Protoc. 2010_x000D_ May;2010(5):pdb.prot5423._x000D_ _x000D_ 3: Fuhrmann-Stroissnigg H, Santiago FE, Grassi D, Ling Y, Niedernhofer LJ,_x000D_ Robbins PD. SA-β-Galactosidase-Based Screening Assay for the Identification of_x000D_ Senotherapeutic Drugs. J Vis Exp. 2019 Jun 28;(148)._x000D_ _x000D_ 4: Lu G, Yi J, Gubas A, Wang YT, Wu Y, Ren Y, Wu M, Shi Y, Ouyang C, Tan HWS,_x000D_ Wang T, Wang L, Yang ND, Deng S, Xia D, Chen RH, Tooze SA, Shen HM. Suppression_x000D_ of autophagy during mitosis via CUL4-RING ubiquitin ligases-mediated WIPI2_x000D_ polyubiquitination and proteasomal degradation. Autophagy. 2019_x000D_ Nov;15(11):1917-1934._x000D_ _x000D_ 5: Ghéczy N, Küchler A, Walde P. Proteinase K activity determination with_x000D_ β-galactosidase as sensitive macromolecular substrate. Anal Biochem. 2016 Nov_x000D_ 15;513:54-60._x000D_ _x000D_ 6: Ullah K, Chen S, Lu J, Wang X, Liu Q, Zhang Y, Long Y, Hu Z, Xu G. The E3_x000D_ ubiquitin ligase STUB1 attenuates cell senescence by promoting the_x000D_ ubiquitination and degradation of the core circadian regulator BMAL1. J Biol_x000D_ Chem. 2020 Apr 3;295(14):4696-4708._x000D_ _x000D_ 7: Srinivas S, Watanabe T, Lin CS, William CM, Tanabe Y, Jessell TM, Costantini_x000D_ F. Cre reporter strains produced by targeted insertion of EYFP and ECFP into the_x000D_ ROSA26 locus. BMC Dev Biol. 2001;1:4._x000D_ _x000D_ 8: Tian S, Zhang Z, Chen J, Du M, Li Z, Yang H, Ji X, He Z. Digital analysis_x000D_ with droplet-based microfluidic for the ultrasensitive detection of β-gal and_x000D_ AFP. Talanta. 2018 Aug 15;186:24-28._x000D_ _x000D_ 9: Gong H, Zhang B, Little G, Kovar J, Chen H, Xie W, Schutz-Geschwender A,_x000D_ Olive DM. beta-Galactosidase activity assay using far-red-shifted fluorescent_x000D_ substrate DDAOG. Anal Biochem. 2009 Mar 1;386(1):59-64._x000D_ 10: Zhang H. (1-(2-(β-Galactopyranosyloxy)propyl)-4,7,10-tris(carboxymethyl)-1,4_x000D_ ,7,10-tetraazacyclododecane) gadolinium(III). 2008 Jan 14 [updated 2008 Feb 20]._x000D_ In: Molecular Imaging and Contrast Agent Database (MICAD) [Internet]. Bethesda_x000D_ (MD): National Center for Biotechnology Information (US); 2004€“2013. _x000D_ _x000D_ Products Related to High Sensitivity beta-Galactosidase Substrate (HSPG): Chemicals

• Short Description:

  Catalog Number: B2010525 (100 mg)_x000D_ High Sensitivity Beta-Galactosidase Substrate (HSPG) is a highly pure ultra high extinction coefficient substrate for beta-galactosidase enzymatic reagents that surpasses all traditional substrates such as ONPG and CPRG. This product has been used as substrate in the development of ultra-sensitive beta-galactosidase-based assays. It has also been used in a wide array of other biochemical and immunological applications. Custom bulk amounts of this product are available upon request._x000D_ _x000D_

• Weight:

  0.15

• Length:

  2

• Width:

  0.5

• Height :

  0.5