Alpha Complementation Kit Beta-galactosidase
For Laboratory Research Only. Not for Clinical or Personal Use.
- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Alpha Complementation Kit Beta-galactosidase
Description:
Alpha Complementation Kit Beta-galactosidase _x000D__x000D_ _x000D_
_x000D__x000D_ _x000D_Catalog # _x000D_K2010002 _x000D__x000D_ _x000D_Content _x000D__x000D_ _x000D_- _x000D_
- Enzyme Acceptor: 2.5 mg lyophilized powder _x000D_
- Enzyme Donor: 0.25 mg lyophilized powder _x000D_
- Enzyme Donor Stabilization Buffer: 15 mL _x000D_
- Enzyme Acceptor Stabilization Buffer: 15 mL _x000D_
- Beta-galactosidase Chromogenic Substrate: 25 mg _x000D_
- Beta-galactosidase Chromogenic Substrate Buffer: 15 mL _x000D_
- Detailed Instructions _x000D_
_x000D_ _x000D_Storage _x000D_-20°C. Avoid repeated freeze/thaw cycles. _x000D__x000D_ _x000D_Supplied as _x000D_Research Kit _x000D__x000D_ _x000D_Buffer _x000D_Contains stabilization buffers _x000D__x000D_ _x000D_Purity _x000D_All enzymes are > 95% pure _x000D__x000D_ _x000D_Application _x000D_Alpha complementation kit, cloned enzyme donor immunoassays, ELISA. _x000D__x000D_ _x000D_Keywords _x000D_Beta-galactosidase Alpha Peptide, Beta-galactosidase Enzyme Donor, Beta-galactosidase Omega Domain, Enzyme Acceptor, Alpha Complementation, LacZ, beta galactosidase fragment sales, enzyme donor and enzyme acceptor active fragments _x000D__x000D_ _x000D_Enzymes Source _x000D_Recombinant _x000D__x000D_ _x000D_ _x000D_Related products _x000D_Beta-galactosidase Enzyme Donor (Alpha Peptide), Stabilization Buffer for Enzyme Acceptor, Beta-galactosidase Enzyme Acceptor (Omega Domain), Beta-galactosidase Enzyme Acceptor Stabilization Buffer, Beta-galactosidase Chromogenic Substrate. _x000D_About Beta-galactosidase Alpha Complementation
_x000D_Beta-galactosidase Alpha-Complementation is a biochemical phenomenon first documented by Agnes Ullmann, while working in the lab of François Jacob and Jacques Monod. By means of molecular cloning, the native E. coli β-galactosidase enzyme can be split in two inactive fragments of different sizes. The smaller fragment, known as the alpha-peptide or enzyme donor, is about 100 amino residues in length and is inactive on its own (incapable of hydrolyzing a β-galactosidase substrate). The larger fragment, known as the omega fragment or enzyme acceptor, is about 900 amino residues in length and is also inactive on its own. Upon mixing the enzyme donor with the enzyme acceptor, the β-galactosidase enzyme is reconstituted and is now capable of hydrolyzing colorimetric substrates such as ONPG.
_x000D_Both enzyme donor and enzyme acceptor can be cloned and expressed in special E. coli strains to yield highly pure, zero-background enzyme fragments (i.e. an enzyme donor and enzyme acceptor without measurable catalytic activities, when assayed individually). Interestingly, it was discovered that various analytes can be conjugated to the enzyme donor moiety and the enzyme donor-enzyme acceptor association modulated by an analyte-binding molecule (such as an antibody). As a result, an alpha-complementation-based assay can be developed.
_x000D_ Download Alpha Complementation Product Line Brochure._x000D_References
_x000D_- _x000D_
- Kras, E. (2019). Beta-galactosidase: properties, structure and functions. New York: Nova Science Publishers. _x000D_
- Arndt, T. (2017). Cloned Enzyme Donor Immunoassay. Lexikon Der Medizinischen Laboratoriumsdiagnostik, 1–2. _x000D_
- Jeon, S. I., Yang, X., and Andrade, J. D. (2004). Modeling of homogeneous cloned enzyme donor immunoassay. Analytical Biochemistry, 333(1), 136–147. _x000D_
- Tachi, T., Kaji, N., Tokeshi, M., and Baba, Y. (2009). Microchip-based Homogeneous Immunoassay Using a Cloned Enzyme Donor. Analytical Sciences, 25(2), 149–151. _x000D_
- Khanna, P. L., and Worthy, T. E. (1993). CEDIA: A Recombinant-Based Homogeneous Enzyme Immunoassay. _x000D_
Short Description:
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