Human ATG4B (Cysteine protease ATG4B) ELISA Kit

Human ATG4B (Cysteine protease ATG4B) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ATG4B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ATG4B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ATG4B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ATG4B in the samples is then determined by comparing the OD of the samples to the standard curve.
• Gene Aliases: HsAPG4B; APG4B; AUTL1
• Field of Research: Enzyme & Kinase.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 20 ng/mL
• Sample Type: Serum, plasma, tissue homogenates
• Detection Range: 0.32-20 ng/mL
• Sensitivity: 0.096 ng/mL