Human M-CSF Protein

CAT: 0749-PRP1937-01Size: 1 mgDry Ice: NoHazardous: No
CAT#:0749-PRP1937-01Size:1 mg
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Background
M-CSF is a potent hematopoietic factor produced by a variety of cells, including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival for blood monocytes, tissue macrophages, and their respective progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness and fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis
Description
M-CSF is a potent hematopoietic factor produced by a variety of cells, including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts.
Product Name Alternative
Macrophage Colony-Stimulating Factor 1; CSF-1; M-CSF; MCSF; Lanimostim; CSF1
Gene ID
1435
Accession Number
P09603-3
Tag
M-CSF
Type
Cytokines and proteins
Applications
No tag
Purity
> 95% as determined by SDS-PAGE
Activity
Measured by its ability to induce proliferation in M-NFS-60 cells. The ED50 for this effect is 0.7344 ng/mL.
Molecular Formula
19 kDa
Molecular Weight
19 kDa
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Endotoxin :< 1 EU per μg of the protein as determined by the LAL method.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Lyophilized protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.
Protein Length
The recombinant Human M-CSF consists of 158 amino acids and predicts a molecular mass of 19 kDa.
Formulation
Lyophilized from sterile 20 mM Tris-HCl, 150mM NaCl, pH8.0.
Applications Notes
Human
Other Product Names
Macrophage Colony-Stimulating Factor 1; CSF-1; M-CSF; MCSF; Lanimostim; CSF1
Recommended Usage
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously
Preparation
E.coli
Accession Number URL
https://www.uniprot.org/uniprotkb/P09603/entry#P09603-3
AA Sequence
Amino acid sequence derived from Human M-CSF (Glu33-Ser190) (P09603-3) was expressed.

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