Human M-CSF Protein

• Background:

  M-CSF is a potent hematopoietic factor produced by a variety of cells, including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival for blood monocytes, tissue macrophages, and their respective progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness and fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis

• Description:

  M-CSF is a potent hematopoietic factor produced by a variety of cells, including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts.

• Product Name Alternative:

  Macrophage Colony-Stimulating Factor 1; CSF-1; M-CSF; MCSF; Lanimostim; CSF1

• Gene ID:

  1435

• Accession Number:

  P09603-3

• Tag:

  M-CSF

• Type:

  Cytokines and proteins

• Applications:

  No tag

• Purity:

  > 95% as determined by SDS-PAGE

• Activity:

  Measured by its ability to induce proliferation in M-NFS-60 cells. The ED50 for this effect is 0.7344 ng/mL.

• Molecular Formula:

  19 kDa

• Molecular Weight:

  19 kDa

• Precautions:

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Features & Benefits:

  Endotoxin :< 1 EU per μg of the protein as determined by the LAL method.

• Shipping Conditions:

  Gel pack with blue ice.

• Storage Conditions:

  Lyophilized protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

• Protein Length:

  The recombinant Human M-CSF consists of 158 amino acids and predicts a molecular mass of 19 kDa.

• Formulation:

  Lyophilized from sterile 20 mM Tris-HCl, 150mM NaCl, pH8.0.

• Applications Notes:

  Human

• Other Product Names:

  Macrophage Colony-Stimulating Factor 1; CSF-1; M-CSF; MCSF; Lanimostim; CSF1

• Recommended Usage:

  Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously

• Preparation:

  E.coli

• Accession Number URL:

  https://www.uniprot.org/uniprotkb/P09603/entry#P09603-3

• AA Sequence:

  Amino acid sequence derived from Human M-CSF (Glu33-Ser190) (P09603-3) was expressed.