Mouse M-CSF protein

Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte / macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M -CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.

Mouse M-CSF protein, expressed in E. coli

CSF-1, M-CSF, Lanimostim, CSF1

1436

P07141-1

No-tag

No tag

> 99 % as determined by SDS-PAGE

Measured by its ability to induce proliferation in M-NFS-60 cells. The ED50 for this effect is 0.05417 ng/mL.

27.4 kDa

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

The product is shipped at ambient temperature.

Lyophilized Mouse M-CSF protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

The Recombinant Mouse M-CSF protein consists of 241 amino acids and predicts a molecular mass of 27.4 KDa

Lyophilized from sterile 20 mM Tris with 150 mM NaCl, pH 7.4.

Mouse

CSF-1, M-CSF, Lanimostim, CSF1

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse M-CSF protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

E. coli

https://www.uniprot.org/uniprotkb/P07141/entry#function