Anti-Measles Phosphoprotein Antibody

Anti-Measles Phosphoprotein Antibody

• Description: Mouse Monoclonal Antibody specific to Measles Phosphoprotein
• Product Name Alternative: Protein P
• CAS Number: 9007-83-4
• Gene Name: P/V
• NCBI Gene ID: P/V
• UniProt: P35974
• Host: Mouse
• Reactivity: Measles Virus
• Immunogen: Recombinant Measles virus phosphoprotein.
• Target Antigen: Phosphoprotein
• Target: Measles Phosphoprotein
• Clonality: Monoclonal
• Isotype: IgG1
• Type: Antibody
• Applications: WB, ICC, ELISA
• Field of Research: Infectious Disease
• Purification Method: Purified by Protein A affinity chromatography
• Dilution: Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 ug/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.
• Format: Purified
• Form: Lyophilized
• Buffer: Tris Buffer
• Function: Essential component of the RNA polymerase and the nascent chain assembly complex. Also required during RNA synthesis.
• Additionnal Information: ELISA: use at 0.1-1.0ug/ml
  
  Immunoblotting:
  
  User should use at 1-5ug/ml. A band of ~70kDa is detected
  
  Immunocytochemistry: use at 2- 10ug/ml on virus-infected cells.
  
  These are recommended concentrations.
  
  End user should determine optimal concentrations for their applications.
• Storage Conditions: This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
• Specificity: This antibody reacts with Measles virus phosphoprotein.
• Formulation: Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.
• Target Background: Measles virus (MeV) is an enveloped RNA virus in the Morbillivirus genus of the Paramyxoviridae family. Its non- segmented, negative-sense, single- stranded RNA genome is encapsidated by the viral nucleoprotein (N) within a helical nucleocapsid. Transcription and replication are carried out on this N:RNA complex by the viral polymerase complex which consists of two components, the large protein (L) and the phosphoprotein (P). MeV N consists of two regions: a structured N-terminal moiety, NCORE (aa 1–400), which contains all the regions necessary for self-assembly and RNA-binding, and a C-terminal domain, NTAIL(aa 401–525) that is intrinsically unstructured and is exposed at the surface of the viral nucleocapsid.