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Mini Sample Human LEP (Leptin) ELISA Kit

CAT: 0763-E-MSEL-H0003-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-MSEL-H0003-01Size:24 Tests
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Abbreviation
LEP
UniProt
P41159
Cross Reactivity
This kit recognizes LEP in samples.No significant cross-reactivity or interference between LEP and analogues was observed.
Target
LEPD; OB; OBS; Obesity Homolog
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Cardiovascular; Metabolism; Neuroscience; Stem cells; Signal transduction
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LEP . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LEP , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of LEP . You can calculate the concentration of LEP in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
39.06-2500 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
14.09pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human LEP concentrations in serum, plasma and other biological fluids.

UniProtKB · P41159

Leptin

LEP_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P41159
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
LEP
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
O15158, Q56A88
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Disease

Diabetes mellitusDisease variantObesity

PTM

Disulfide bond

Cellular component

Secreted

Domain

Signal

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