Mini Sample Mouse LEP (Leptin) ELISA Kit

LEP

P41160

This kit recognizes Mouse LEP in samples.No significant cross-reactivity or interference between Mouse LEP and analogues was observed

LEP

Sandwich-ELISA

ELISA

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal transduction; Stem cells

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LEP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LEP. You can calculate the concentration of Mouse LEP in the samples by comparing the OD of the samples to the standard curve.

3 h 30 min

Serum, plasma and other biological fluids

0.63-40 ng/mL

Both intra-CV and inter-CV are < 10%.

80%-120%

0.38 ng/mL

Ice bag

2-8°C,12 months

https://academic.oup.com/biomedgerontology/article-abstract/76/3/393/5813438

https://www.sciencedirect.com/science/article/pii/S0891584918314394

https://www.sciencedirect.com/science/article/pii/S0269749120363867

https://www.mdpi.com/1042904

https://www.sciencedirect.com/science/article/pii/S0045653519329017

https://www.mdpi.com/1422-0067/21/14/5121

https://www.tandfonline.com/doi/abs/10.1080/13813455.2021.1894178

https://www.mdpi.com/2218-273X/10/10/1454

https://pubs.rsc.org/en/content/articlelanding/2020/fo/d0fo01405b

https://www.sciencedirect.com/science/article/pii/S1756464619300854

https://www.sciencedirect.com/science/article/pii/S001429992030296X

https://www.sciencedirect.com/science/article/pii/S0024320520301909

Mouse