Mouse TF (Transferrin) ELISA Kit

CAT: 0763-E-EL-M1184-01Size: 24 TestsDry Ice: NoHazardous: No

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CAT#:0763-E-EL-M1184-01Size:24 Tests

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Abbreviation

UniProt

Q921I1

Cross Reactivity

This kit recognizes Mouse TF in samples. No significant cross-reactivity or interference between Mouse TF and analogues was observed

Target

Beta-1 metal-binding globulin; ; Serotransferrin; Siderophilin; TF; Transferrin

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TF. You can calculate the concentration of Mouse TF in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse TF concentrations in Serum, plasma and other biological fluids.

UniProtKB · Q921I1

Serotransferrin

TRFE_MOUSE · Mus musculus

View on UniProt ↗

Primary accession: Q921I1
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: Tf
Protein existence: 1: Evidence at protein level
Organism: Mus musculus (Mouse)
Taxonomy ID: 10090
Alternative names: Transferrin
EC number: —
Processing: Precursor
Secondary accessions: O35421, Q3UBW7, Q58E69, Q61803, Q62357, Q62358, Q62359, Q63915, Q64515, Q8VII5, Q922C0

Protein keywords

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoproteinMethylationPhosphoprotein

Biological process

Ion transportIron transportTransport

Ligand

IronMetal-binding

Domain

RepeatSignal

Cellular component

Secreted

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