Transferrin ELISA Kit (Mouse)

• Gene Name:

  Transferrin

• Gene Aliases:

  H, HP, Tf, Tfn, hpx, Cd176, AI266983

• Gene ID:

  22041

• Reactivity:

  Mouse

• Target:

  Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric, OD450 nm

• Assay Principle:

  Aviva Systems Biology Transferrin ELISA Kit (Mouse) (OKIA00115) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Serotransferrin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Serotransferrin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serotransferrin captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  3.125 ng/ml - 100 ng/ml

• Sensitivity:

  0.875 ng/ml

• Homology:

  Mouse

• Format:

  In Solution

• Reconstitution:

  Store as indicated in product manual.

• Components:

  Component Amount Anti-Mouse Transferrin Microplate 96 Wells (12 x 8 Well Strips) 20X Diluent Concentrate 50 mL 20X Wash Solution 50 mL 100X Enzyme-Antibody Conjugate 150 uL Chromogen-Substrate Solution 12 mL Stop Solution 12 mL Mouse Transferrin Calibrator 1 Component Amount Component Amount Anti-Mouse Transferrin Microplate 96 Wells (12 x 8 Well Strips) Anti-Mouse Transferrin Microplate 96 Wells (12 x 8 Well Strips) 20X Diluent Concentrate 50 mL 20X Diluent Concentrate 50 mL 20X Wash Solution 50 mL 20X Wash Solution 50 mL 100X Enzyme-Antibody Conjugate 150 uL 100X Enzyme-Antibody Conjugate 150 uL Chromogen-Substrate Solution 12 mL Chromogen-Substrate Solution 12 mL Stop Solution 12 mL Stop Solution 12 mL Mouse Transferrin Calibrator 1 Mouse Transferrin Calibrator 1

• Notes:

  Formerly GWB-842102

• NCBI Gene Symbol:

  TRF

• Protein Name:

  Serotransferrin

• Gene Name URL:

  TRF

• CAS Number:

  7732-18-5