CD274, PD-L1

• Background:

  Programmed cell death ligand 1 (PD-L1) also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1) is a type 1 transmembrane protein involved in the regulation of cellular and humoral immune responses. The interaction of PD-L1 with its receptor PD-1 provides both stimulatory and inhibitory signals in regulating T cell activation and tolerance during pregnancy, tissue allograft, autoimmune disease and malignant transformation. The binding of PD-L1 to PD-1 induces apoptosis or exhaustion in activated T cells, and blockade of this interaction has been shown to enhance the antitumor activity of T cells. PD-L1 is frequently over expressed in placenta, and many Human tumours such as melanoma, diffuse large B-cell lymphoma, and carcinomas of the lung, bladder, breast, kidney and colon.

• Synonyms:

  Human CD274, PD-L1.

• UniProt:

  Q9NZQ7 (PD1L1_Human)

• Host:

  Rabbit

• Species Reactivity:

  Human

• Isotype:

  IgG

• Clone:

  ZR3

• Type:

  Primary Antibodies

• Source:

  Rabbits were immunized with KLH-conjugated linear peptide from Human PD-L1

• Applications:

  IHC (frozen), IHC (paraffin)

• Field of Research:

  Cell Biology, Immunology, Oncology

• Assay Principle:

  IHC(C, P)

• Form:

  Antibody solution in stabilizing phosphate buffer pH 7,3; Contains 0,09 % sodium azide**

• Precautions:

  *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in Humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

• References & Citations:

  1. Ishida, Agata, Shibahara et al. (1992) Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO Journal, Vol. 11, Issue 11, pp. 3887-95 _x000D_ 2. Wang W, Li H, Xu F, DU H, Li X, Yi J, Wang G, Luan X. Xi Bao (2016) IFN-gamma up-regulates PD-L1 expression in Human placenta mesenchymal stem cells and enhances cell ability to induce the differentiation of IL-10+ T cells from cord blood- and peripheral blood-derived T cells. Yu Fen Zi Mian Yi Xue Za Zhi. 32(2):191-5. Chinese PMID 26927379_x000D_ 3. Benjamin J. Chen, Bjoern Chapuy, Jing Ouyang, et al. (2013) PD-L1 Expression Is Characteristic of a Subset of Aggressive B-cell Lymphomas and Virus-Associated Malignancies. Clinical Cancer Research, 19; 3462-73. CCR-13-0855

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  The antibody may be stored at +4°C; For prolonged storage prepare appropriate aliquots and store at or below -20°C; Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance or the concentration of the product