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Recombinant Human SIRT1

CAT: 0710-P0459-01Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0710-P0459-01Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
EC 3.5.1, S. cerevisiae, homolog) 1, SIR2alpha, SIR2L1, SIR2-like protein 1, SIRT1, Sirtuin 1
UniProt
Q96EB6
Expression Region
510-747
Host
E.Coli
Tag
N-terminal His Tag
Applications
Western Blot, ELISA
Field of Research
Neuroscience, Stem Cells, Metabolism, Developmental biology
Purity
Greater than 90% as determined by SDS-PAGE.
Form
Lyophilized powder
Reconstitution
Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.
Molecular Weight
27.9 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.
Formulation
Lyophilized from a 0.2 μm filtered solution of 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4.
Organism Species
Human

UniProtKB · Q96EB6

NAD-dependent protein deacetylase sirtuin-1

SIR1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q96EB6
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SIRT1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
hSIRT1
EC number
2.3.1.286
Processing
—
Secondary accessions
Q2XNF6, Q5JVQ0, Q9GZR9, Q9Y6F0
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationMethylationPhosphoproteinS-nitrosylationUbl conjugation

Coding sequence diversity

Alternative splicing

Biological process

ApoptosisBiological rhythmsDifferentiationHost-virus interactionMyogenesisTranscriptionTranscription regulation

Cellular component

CytoplasmMitochondrionNucleus

Molecular function

Developmental proteinTransferase

Ligand

Metal-bindingNADZinc

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