Human Lactotransferrin, LTF ELISA Kit

Lactotransferrin, also known as Lactoferrin, Talalactoferrin, and LTF, is a secreted protein that belongs to the transferrin family. Transferrins are iron binding transport proteins that can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration. Lactoferroxins A, B, and C have opioid antagonist activity. Lactoferrin A shows a preference for mu-receptors, while lactoferricin B and lactoferricin C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors. Lactoferrin / LTF is a globular glycoprotein that is widely represented in various secretory fluids, such as milk, saliva, tears, and nasal secretions. Lactoferrin / LTF is also present in secondary granules of PMN and is secreted by some acinar cells. Lactoferrin / LTF can be purified from milk or produced recombinantly. Human colostrum has the highest concentration, followed by human milk, then cow milk. Lactoferrin / LTF is one of the components of the immune system of the body; it has antimicrobial activity (bacteriocide, fungicide) and is part of the innate defense, mainly at mucose. In particular, lactoferrin provides an antibacterial activity to human infants. Lactoferrin interacts with DNA and RNA, polysaccharides and heparin, and shows some of its biological functions in complexes with these ligands.

Lactotransferrin

GIG12; LF

LTF

4057

P02788

Lactoferrin

Cancer, Cardiovascular, Signal transduction

Sandwich

Concentration | O.D. | Average | Corrected ------------------------------------------ 5000 pg/ml | 2.482 | 2.368 | 2.288 2.255 2500 pg/ml | 1.203 | 1.228 | 1.148 1.253 1250 pg/ml | 0.654 | 0.621 | 0.541 0.589 625 pg/ml | 0.373 | 0.354 | 0.274 0.336 312.5 pg/ml | 0.204 | 0.218 | 0.138 0.232 156.25 pg/ml | 0.134 | 0.136 | 0.056 0.138 78.125 pg/ml | 0.112 | 0.111 | 0.031 0.11 0 pg/ml | 0.078 | 0.08 | 0 0.083

3.5h

Serum, plasma, cell culture supernates

78.125-5000 pg/mL

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 169 | 8.2 | 4.9 Intra-assay | 2 | 20 | 604 | 25.5 | 4.2 Intra-assay | 3 | 20 | 2517 | 218 | 8.7 Inter-assay | 1 | 20 | 167 | 10.4 | 6.3 Inter-assay | 2 | 20 | 603 | 45 | 7.5 Inter-assay | 3 | 20 | 2493 | 176 | 7.1

20.95 pg/mL

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail

2-8°C

Human