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Human Complement Component C1Q Receptor, CD93 ELISA Kit

CAT: 1360-BPE256-01Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:1360-BPE256-01Size:96 Tests
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Background
CD93 or C1q receptor 1 (C1qR) is an about 120 kDa O-sialoglycoprotein that within the hematopoietic system is selectively expressed on cells of the myeloid lineage. CD93/C1qR is a highly glycosylated transmembrane protein expressed on monocytes, neutrophils, endothelial cells, and stem cells. CD93 was originally identified as a myeloid cell-surface marker and subsequently associated with an ability to modulate phagocytosis of suboptimally opsonized immunoglobulin G and complement particles in vitro. CD93/C1qR, a receptor expressed during early B-cell development, is reinduced during plasma-cell differentiation. High CD93/CD138 expression was restricted to antibody-secreting cells both in T-dependent and T-independent responses as naive, memory, and germinal-center B cells remained CD93-negative. CD93 was expressed on (pre) plasmablasts/plasma cells, including long-lived plasma cells that showed decreased cell cycle activity, high levels of isotype-switched Ig secretion, and modification of the transcriptional network. CD93 is important for the maintenance of plasma cells in bone marrow niches.
Product Name Alternative
Complement component C1q receptor
Synonyms
C1QR1; MXRA4; C1qR; C1qRp; CDw93; dJ737E23.1; ECSM3
Gene Name
CD93
Gene ID
22918
UniProt
Q9NPY3
Target
CD93
Field of Research
Cardiovascular, Immunology, Stem cells
Assay Type
Sandwich
Assay Protocol
Concentration | O.D. | Average | Corrected ------------------------------------------ 2000 pg/ml | 2.147 | 2.198 | 2.116 2.249 1000 pg/ml | 1.176 | 1.233 | 1.151 1.29 500 pg/ml | 0.699 | 0.707 | 0.625 0.716 250 pg/ml | 0.376 | 0.39 | 0.308 0.405 125 pg/ml | 0.222 | 0.238 | 0.156 0.255 62.5 pg/ml | 0.161 | 0.161 | 0.079 0.162 31.25 pg/ml | 0.126 | 0.119 | 0.037 0.113 0 pg/ml | 0.078 | 0.082 | 0 0.087
Assay Performance Time
3.5h
Sample Type
Serum, plasma, cell culture supernates
Detection Range
31.25-2000 pg/mL
Precision
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 137 | 9.5 | 6.9 Intra-assay | 2 | 20 | 546 | 29.1 | 5.3 Intra-assay | 3 | 20 | 1561 | 45.4 | 2.9 Inter-assay | 1 | 20 | 136 | 10.8 | 7.9 Inter-assay | 2 | 20 | 543 | 37.6 | 6.9 Inter-assay | 3 | 20 | 1578 | 93.5 | 5.9
Sensitivity
10.56 pg/mL
Components
Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail
Storage Temperature
2-8°C
Species
Human
Mass
68560