Human Complement Component C5A ELISA Kit

C5a is a protein fragment released from complement component C5. This 74 amino acid peptide in humans is generated by the cleavage of C5a convertase on the C5 α-chain during the classical, alternative, and lectin pathways of complement activation. The structure of C5a includes a core region consisting of four, anti-parallel alpha-helices held together by three disulfide linkages and a structured C-terminal tail, and C5a is rapidly metabolised by carboxypeptidase B to a 73 amino acid low activity form, C5a des-Arg. C5a is an extremely potent proinflammatory mediator, as well as a potent chemotactic factor for neutrophils and other leukocytes. It causes histamine release, increases in vascular permeability, induces several cytokines production from leukocytes, enhances neutrophil-endothelial cell adhesion, and augments the humoral and cell-mediated immune response. C5a is quickly metabolised by carboxypeptidases, forming the less potent C5adesArg. Acting via a classical G protein-coupled receptor, CD88, C5a and C5adesArg exert a number of effects essential to the innate immune response, while their actions at the more recently discovered non-G protein-coupled receptor, C5L2 (or GPR77), remain unclear. The widespread expression of C5a receptors throughout the body allows C5a to elicit a broad range of effects. Thus, C5a has been found to be a significant pathogenic driver in a number of immuno-inflammatory diseases, making C5a inhibition an attractive therapeutic strategy. C5a is a strong chemoattractant and is involved in the recruitment of inflammatory cells such as neutrophils, eosinophils, monocytes, and T lymphocytes, in activation of phagocytic cells and release of granule-based enzymes and generation of oxidants, all of which may contribute to innate immune functions or tissue damage. Accordingly, the anaphylatoxin C5a is implicated in a variety of diseases such as rheumatoid arthritis, systemic lupus erythematosus, reperfusion injury, Alzheimer's disease, and sepsis.

Complement Component C5a

C5; CPAMD4; CPAMD4; MGC142298

C5a

Immunology, Metabolism, Cell biology, Signal transduction

Sandwich

Concentration | O.D. | Average | Corrected ------------------------------------------ 2000 pg/ml | 2.495 | 2.453 | 2.365 2.411 1000 pg/ml | 1.328 | 1.338 | 1.25 1.349 500 pg/ml | 0.621 | 0.586 | 0.498 0.551 250 pg/ml | 0.364 | 0.361 | 0.273 0.358 125 pg/ml | 0.247 | 0.258 | 0.17 0.269 62.5 pg/ml | 0.138 | 0.143 | 0.055 0.148 31.25 pg/ml | 0.118 | 0.12 | 0.032 0.122 0 pg/ml | 0.087 | 0.088 | 0 0.089

3.5h

Serum, plasma, cell culture supernates

31.25-2000 pg/mL

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 238 | 14 | 5.9 Intra-assay | 2 | 20 | 529 | 15.2 | 2.9 Intra-assay | 3 | 20 | 1080 | 22.6 | 2.1 Inter-assay | 1 | 20 | 240 | 15.5 | 6.5 Inter-assay | 2 | 20 | 526 | 21.1 | 4 Inter-assay | 3 | 20 | 1084 | 37.4 | 3.5

12.81 pg/mL

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail

2-8°C