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TWEAK (soluble) (human), (recombinant)

CAT: 1106-ALX-522-021-C010Size: 10 µgDry Ice: NoHazardous: No
CAT#:1106-ALX-522-021-C010Size:10 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Apo-3L, TNFSF 12
Certification
RUO
UniProt
O43508
Source
Produced in E. coli . The extracellular domain of human TWEAK (aa 106-249) is fused at the N-terminus to a linker peptide (11 aa) and a FLAG ® -tag.
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
0.1 mg/mL after reconstitution.
Endotoxin
<0.1EU/μg purified protein (LAL test; Bio Whittaker) .
Purity
≥90% (SDS-PAGE)
Activity
Kills TWEAK sensitive cells.
Reconstitution
Reconstitute with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
Molecular Weight
~18-20kDa (SDS-PAGE) .
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
Historical data has shown that TWEAK has a pro-apoptotic effect on several target cell lines, and it induces angiogenesis and proliferation in endothelial cells. FLAG is a registered trademark of Sigma-Aldrich Co.
Specificity
Binds to human and mouse Fn14 (TWEAK-R) .
Formulation
Lyophilized. Contains PBS.
Applications Notes
ELISA: binds to FN14 receptor at 10-100 ng/ml.

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