MMP-3 fluorogenic substrate

CAT: 1106-BML-P278-0100Size: 0.1 mgDry Ice: NoHazardous: No

CAT#:1106-BML-P278-0100Size:0.1 mg

Selected

PRICE

AVAILABILITY: InStock

24/48H Stock Items & 2 to 6 Weeks non Stock Items.

1 / 1

Certification

RUO

Sequence

5′-FAM-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys (TQ2W) -NH 2

Stability

As indicated on product label or CoA when stored as recommended.

Purity

≥90% (HPLC)

Solubility

Soluble in DMSO (5mM) .

Molecular Weight

2156.9

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Notes

This substrate offers key advantages over other MMP-3 substrates. Emission at the green end of the spectrum avoids the interference at lower wavelengths often exhibited by screening compounds, and by substances commonly found in biological samples and tissue culture medium. MMP substrate peptides display poor aqueous solubility, often with K m s near or above their limits of solubility, making enzyme and inhibitor kinetics difficult. MMP-3 K m (~0.64μM) for this substrate is below its solubility limit (~2μM in assay buffer), allowing for substrate concentrations higher than the K m , a condition generally desirable in endpoint assays. In addition to the efficient binding as exhibited by low K m , this substrate is avidly cleaved by MMP-3, with a k cat / K m of ~6.5 x 10 7 M -1 sec -1 . The high k cat / K m and the ultra-strong fluorescence of this substrate allow for substrate concentrations much lower than the K m , a condition generally desirable in inhibitor screening/kinetics assays.

Appearance

Lyophilized orange or red solid.

Applications Notes

Useful for inhibitor screening, kinetic analysis, and cellular activity assay.

Related Products

Catalog	Name
BML-P128-0001	MMP substrate (fluorogenic)
BML-P131-0001	MMP substrate α (fluorogenic)
ALX-260-137-M005	HDAC substrate (fluorogenic)
BML-P126-0001	OMNIMMP® fluorogenic substrate
BML-P235-0001	ADAM substrate (fluorogenic)
BML-P139-0050	OMNICATHEPSIN®fluorogenic substrate
BML-P139-0010	OMNICATHEPSIN®fluorogenic substrate
BML-P132-0500	TACE substrate (fluorogenic)
ALX-260-137-M001	HDAC substrate (fluorogenic)
ALX-260-087-M001	Proteasome substrate IV (fluorogenic)

Alternative Products

Catalog	Name
MBS566476-01	MMP-3 fluorogenic substrate
sc-471138	MMP substrate α (fluorogenic)
B3262-5	MMP Substrate, Fluorogenic
T83759-01	MMP-3 Fluorogenic Substrate TFA
5-01558	MMP-3 Substrate I, fluorogenic
MBS8246789-01	MMP-3 Substrate I fluorogenic
MBS8246791-01	MMP-8 Substrate fluorogenic
B2014068	MMP-13 Substrate, Fluorogenic
MBS8246784-01	MMP Substrate I fluorogenic
B2013672	MMP-2 Substrate Fluorogenic

MMP-3 fluorogenic substrate

Related Products

Alternative Products

Popular Products