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CD40L, soluble (mouse) (recombinant) set

CAT: 1106-ALX-850-075-KI01Size: 1 SetDry Ice: NoHazardous: No
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CAT#:1106-ALX-850-075-KI01Size:1 Set
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
CD154, TNFSF 5, gp39
Certification
RUO
UniProt
P27548 (CD40L)
Source
Produced in HEK 293 cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG ® -tag.
Applications
ELISA, FUNC
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
CD40L: 0.1 mg/mL after reconstitution.Enhancer: 1 mg/mL after reconstitution.
Endotoxin
<0.1EU/μg purified protein (LAL test; Bio Whittaker) .
Purity
≥90% (SDS-PAGE) .
Reconstitution
Reconstitute CD40L with 100μl sterile water and each vial of enhancer with 50μl of sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
CD40L (Prod. No. ALX-522-070) and Enhancer for Ligands (Prod. No. ALX-804-034) should be incubated together for 30 min at room temperature prior to cell application. Effects are typically best seen with 0.5-10μg/ml of CD40L together with 1-2μg/ml of enhancer. This may vary by cell type and experimental conditions. FLAG is a registered trademark of Sigma-Aldrich Co.
Specificity
CD40L binds to human and mouse CD40 in an ELISA assay.
Formulation
Lyophilized. Contains PBS.
Applications Notes
Functional Application: Stimulates the proliferation of mouse B cells and dendritic cells. The activity of CD40L increases 1’000-fold (stimulation in the ng/ml range) in the presence of the cross-linking enhancer (Prod. No. ALX-804-034) .
Contents
10μg of CD40L, Soluble (mouse) (recombinant) (Prod. No. ALX-522-070) and 2x50μg of Enhancer for Ligands (Prod. No. ALX-804-034) which increases the biological activity of CD40L at least 1’000-fold.

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