CD40L (soluble) (mouse), (recombinant)

CAT: 1106-ALX-522-070-2010Size: 2x 10 μgDry Ice: NoHazardous: No
CAT#:1106-ALX-522-070-2010Size:2x 10 μg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
CD154, TNFSF 5, gp39
Certification
RUO
UniProt
P27548
Source
Produced in HEK 293 cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG ® -tag.
Purification
Purified by multi-step chromatography.
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
0.1 mg/mL after reconstitution.
Endotoxin
<50EU/mg purified protein (LAL test; Associates of Cape Cod) .
Purity
≥90% (SDS-PAGE)
Reconstitution
Reconstitute each tube with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
Molecular Weight
~26kDa (SDS-PAGE) .
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
Historical data has shown that mouse CD40L stimulates the proliferation of mouse B cells and dendritic cells. The activity of rmsCD40L increases 1’000-fold (stimulation in the ng/ml range) in the presence of a cross-linking enhancer (see Set Prod. No. ALX-850-075) . FLAG is a registered trademark of Sigma-Aldrich Co.
Specificity
Binds to human and mouse CD40.
Formulation
Lyophilized. Contains PBS.
Applications Notes
ELISA: binds to human CD40 at 10-500 ng/ml.

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