Caspase-3 (human), (recombinant) (active)

CAT: 1106-ALX-201-059-U025Size: 25 UDry Ice: YesHazardous: No
CAT#:1106-ALX-201-059-U025Size:25 U
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Specifications
≥15,000 U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate DEVD- p NA (Prod. No. ALX-260-033) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
Product Name Alternative
CPP32, Yama, Apopain
Certification
RUO
UniProt
P42574
Source
Produced in E. coli . Contains an N-terminal His-tag.
Reconstitution
Reconstitute to 1U/μl in water.
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Lyophilized.
Applications Notes
Useful in studying enzyme regulation, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity. For a complete caspase-3 assay protocol, please refer to Caspase-3 Fluorometric or Colorimetric Assay Kits (Prod. No. ALX-850-216 or ALX-850-215) .
Quality Control
Routinely tested for its ability to cleave the caspase-3 substrates Ac-DEVD- p NA (Prod. No. ALX-260-033) and Ac-DEVD-AFC (Prod. No. ALX-260-032) .

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