BIOARRAYTM Single-round RNA amplification and biotin labeling system

CAT: 1106-ENZ-42420-10Size: 10 ReactionsDry Ice: YesHazardous: No

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CAT#:1106-ENZ-42420-10Size:10 Reactions

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Certification

RUO

Stability

As indicated on product label or CoA when stored as recommended. Store Box A at -20 ̊C in a non-frost free freezer. Store Box B at ambient condition.

Shipping Conditions

Dry Ice

Storage Conditions

-20°C

Quality Control

ARNA yield is ≥23μg aRNA purity range is between an A 260 /A 280 ratio of 1.9 and 2.3 Median aRNA size is equal to or greater than 1200 nt Affymetrix GeneChip criteria: % Present calls ≥ than 40% Scale factors ≤ 3.0 5’/3’ ratios for actin and GAPDH ~ 1.0 ± 0.3

Contents

Box A: 40 μl, dNTP Mix (dN) 10 μl, Promoter Primer (P) 20 μl, First Strand Buffer (FB) 80 μl, DTT (D) 10 μl, Reverse Transcriptase (RT) 10 μl, RNase Inhibitor (I) 50 μL, DNA Polymerase (DP) 10 μl, RNase H (RH) 150 μL, Second Strand Buffer (SB) 60 μl, IVT Reaction Buffer (RB) 60 μl, 10x Biotin-Labeled Ribonucleotides (B) 60 μl, Enhancer Cocktail (EC) 30 μl, T7 RNA Polymerase (T7) 2.5 mL, Nuclease-free Water (W) Box B: 4.5 ml, cDNA Binding Buffer (BB) 1.2 ml, 5X Wash Solution (WS) 10 cDNA Purification Columns 10 cDNA Elution Tubes 10 Collection Tubes

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BIOARRAYTM Single-round RNA amplification and biotin labeling system

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