BIOARRAY HIGHYIELD®RNA Transcript Labeling Kit (T7)

CAT: 1106-ENZ-42655-40Size: 40 ReactionsDry Ice: YesHazardous: No
CAT#:1106-ENZ-42655-40Size:40 Reactions
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Certification
RUO
Stability
Stable for at least one year after receipt when stored as recommended.
Shipping Conditions
Dry Ice
Storage Conditions
-20°C
Specificity
Yield: Each lot is shown to generate 50 μg of cRNA when transcribed from a double stranded cDNA library generated from 2 μg total RNA (mixed tissue) in a single round of amplification. Incorporation: Each lot is tested to ensure at least 10 % of incorporated nucleotides are biotin labeled. RNase activity: Insignificant amounts of RNase (less than 100 fg RNase A activity in 1 μL of reagent) are detected. % P value and 3’/5’ ratio: The cRNA synthesized by the kit is tested on Affymetrix HU133A 2.0 chip and shown to give > 35% P-calls, with 3’/5’ ratios for both β-actin and GAPDH of < 2.5.
Contents
10X HY Reaction Buffer 10X Biotin-Labeled Ribonucleotides 10X DTT 10X RNase Inhibitor Mix 20X T7 RNA Polymerase

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