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Abl1 (mouse), (recombinant) (His-tag)

CAT: 1106-BML-SE563-0005Size: 5 µgDry Ice: YesHazardous: No
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CAT#:1106-BML-SE563-0005Size:5 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Specifications
820 nmol/min/mg using a peptide substrate, EAIYAAPFAKKK, in 25mM MOPS, pH 7.2, containing 12.5mM β-glycerol-phosphate, 25mM MgCl 2 , 5mM EGTA, 2mM EDTA. Add 0.25mM DTT to assay buffer prior to use.
Product Name Alternative
Abelson murine leukemia viral oncogene homolog 1
Certification
RUO
UniProt
P00520
Source
Produced in insect cells. Produced in a baculovirus expression system.
Sequence
Mouse recombinant Abl1 (aa 27-1123) with N-terminal histidine tag. Mouse Abl1 is 89% identical (91% similar) to human Abl1.
Concentration
0.1 mg/mL
Purity
≥70% (SDS-PAGE)
Molecular Weight
~135kDa
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Applications Notes
Useful tool to study enzyme regulation and kinetics, phosphorylate target substrates, screen for inhibitors.

UniProtKB · P00520

Tyrosine-protein kinase ABL1

ABL1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P00520
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Abl1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
2.7.10.2
Processing
—
Secondary accessions
P97896, Q61252, Q61253, Q61254, Q61255, Q61256, Q61257, Q61258, Q61259, Q61260, Q61261, Q6PCM5, Q8C1X4
Protein keywords

Technical term

3D-structureReference proteome

PTM

AcetylationLipoproteinMyristatePhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicingChromosomal rearrangement

Biological process

ApoptosisAutophagyCell adhesionDNA damageDNA repairEndocytosis

Ligand

ATP-bindingMagnesiumManganeseMetal-bindingNucleotide-binding

Cellular component

CytoplasmCytoskeletonMitochondrionNucleus

Molecular function

DNA-bindingKinaseTransferaseTyrosine-protein kinase

Disease

Proto-oncogene

Domain

SH2 domainSH3 domain

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