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Hoechst 33342 trihydrochloride

CAT: 1568-C04833Size: 25 mgDry Ice: NoHazardous: No
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CAT#:1568-C04833Size:25 mg
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CAS Number
875756-97-1
Synonyms
BisBenzimide H 33342 trihydrochloride; HOE 33342 trihydrochloride
SKU
C04833-25mg
Additionnal Information
Hoechst 33342 trihydrochloride (Bisbenzimide ethoxide trihydrochloride, HOE 33342 trihydrochloride) is a bis-benzimide fluorescent dye that specifically binds to AT-rich sequences in the minor groove of double-stranded DNA. When exposed to UV light, it emits bright fluorescence at Hoechst Red (630–650 nm) and Hoechst Blue (405–450 nm) wavelengths. This dye is used to determine cell cycle status, conduct apoptosis assays, and act as a nuclear marker in microscopy.

Chemical Information

Bisbenzimide ethoxide trihydrochloride

benzimidazole fluorescent dye

CAS Number875756-97-1
PubChem CID16760503
IUPAC Name2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole;trihydrochloride
Molecular FormulaC27H31Cl3N6O
Molecular Weight561.9
Topological Polar Surface Area73.1
Hydrogen Bond Donor Count5
Hydrogen Bond Acceptor Count5
Rotatable Bond Count5
Heavy Atom Count37
Formal Charge0
Complexity664
SMILES
CCOC1=CC=C(C=C1)C2=NC3=C(N2)C=C(C=C3)C4=NC5=C(N4)C=C(C=C5)N6CCN(CC6)C.Cl.Cl.Cl
InChI
InChI=1S/C27H28N6O.3ClH/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33;;;/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31);3*1H
InChIKey
JABNPSKWVNCGMX-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Bisbenzimide ethoxide trihydrochloride
CAS: 875756-97-1
CID: 16760503
Formula: C27H31Cl3N6O
MW: 561.9
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight561.9
Hydrogen Bond Donor Count5
Hydrogen Bond Acceptor Count5
Rotatable Bond Count5
Exact Mass560.162493
Monoisotopic Mass560.162493
Topological Polar Surface Area73.1 Ų
Heavy Atom Count37
Formal Charge0
Complexity664
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count4
Compound Is CanonicalizedYes

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