BID (Cleaved-Arg71) rabbit pAb

BID (Cleaved-Arg71) rabbit pAb

• Description: Domain: Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. function: The major proteolytic product p15 BID allows the release of Cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. PTM: TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products. subcellular location: A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively. subcellular location: Associated with the mitochondrial membrane. subcellular location: Translocates to mitochondria as an integral membrane protein. subcellular location: When uncleaved, it is predominantly cytoplasmic. subunit: Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein Bcl-2. tissue specificity: Isoforms 2 and 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
• Synonyms: BH3-interacting domain death agonist (p22 BID;BID) [Cleaved into: BH3-interacting domain death agonist p15 (p15 BID); BH3-interacting domain death agonist p13 (p13 BID); BH3-interacting domain death agonist p11 (p11 BID)]
• Gene ID: 637
• UniProt: P55957
• Cellular Locus: Cytoplasm. Mitochondrion membrane. Mitochondrion outer membrane. When uncleaved, it is predominantly cytoplasmic..; [BH3-interacting domain death agonist p15]: Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein..; [BH3-interacting domain death agonist p13]: Mitochondrion membrane. Associated with the mitochondrial membrane..; [Isoform 1]: Cytoplasm.; [Isoform 3]: Cytoplasm.; [Isoform 2]: Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively..
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Immunogen: Synthesized peptide derived from human BID (Cleaved-Arg71)
• Clonality: Polyclonal
• Validated Applications: WB, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: WB 1:1000-2000 ELISA 1:5000-20000
• Molecular Weight: 8 22kD
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.