Products for Research Use Only
RegisterLogin

Anti-PKC (α, β, γ) Antibody

CAT: 1549-PM4991Size: 100 µLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:1549-PM4991Size:100 µL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V) . The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (α, β1, βII, and γ) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. The phosphorylation of multiple sites in conventional PKCs regulates their activity. In mast cells, FceRI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKCα and PKCβI respectively. This phosphorylation requires autophosphorylation of serine 657 and 661 in these respective kinases.
Description
Our PKC (α, β, γ) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat PKC (α, β, γ) and is protein g purified. It is great for use in ELISA, WB, ICC.
Short Description
Our PKC (α, β, γ) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat PKC (α, β, γ) and is Protein G purified. It is great for use in WB, ICC.
Synonyms
PKCalpha, PKCbeta, PKCgamma
Gene Name
PRKCA
UniProt
P05129
Reactivity
Chicken, Human, Mouse, and Rat
Immunogen
Clone (M499) was generated from a recombinant human PKCγ that included amino acids residues 499-697.
Target
PKC (α, β, γ)
Clonality
Monoclonal
Clone
M499
Conjugation
Unconjugated
Applications
ELISA, WB, ICC
Field of Research
Cell Signaling, Kinase Signaling, Oncoproteins
Format
Protein G Purified
Buffer
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Molecular Weight
80-82
Precautions
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Shipping Conditions
Blue Ice
Storage Conditions
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Notes
Protein G purified culture supernatant.
WB Dilution
1:1000
Specificity
This antibody detects 80-82kDa* proteins corresponding to the molecular mass of PKCα, PKCβ, and PKCγ on SDS-PAGE immunoblots of mouse brain lysates. Similar results were observed in human and mouse lysates. This antibody detects recombinant PKCα, PKCβ1, and PKCγ, and possibly detects other PKC isoforms as well.
ICC Dilution
1:100
Quality Control
Western blots performed on each lot.
Host or Source
Mouse
Physical Properties
Liquid
Isotype
IgG2a
Immunogen Species
Human
WB Quality Control
Quality Control: WB
RRID
AB_3068486

UniProtKB · P05129

Protein kinase C gamma type

KPCG_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P05129
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PRKCG
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
PKC-gamma
EC number
2.7.11.13
Processing
—
Secondary accessions
B7Z8Q0
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingCalciumMetal-bindingNucleotide-bindingZinc

Biological process

Biological rhythms

Cellular component

Cell membraneCell projectionCytoplasmMembraneSynapseSynaptosome

Disease

Disease variantNeurodegenerationSpinocerebellar ataxia

Molecular function

KinaseSerine/threonine-protein kinaseTransferase

PTM

PhosphoproteinUbl conjugation

Domain

RepeatZinc-finger

Related Products

CatalogName
PM2371Anti-PKCα (Central region) Antibody
PM2171Anti-PKCθ (N-terminal region) Antibody
PM2421Anti-PKCδ (N-terminal region) Antibody
PP1091Anti-PKCα (Ser-657/Tyr-658), Phosphospecific Antibody
PK6540PKC (α, β, γ) Phospho-Regulation Antibody Kit
VP2701Anti-Vav3 Antibody
VP2481Anti-Vav2 Antibody
NP2281Anti-eNOS Antibody
FM0221Anti-Fibronectin Antibody
CM2361Anti-Cdk5 Antibody

Alternative Products

CatalogName
STJ95117Anti-PKC antibody
STJ95128Anti-PKC epsilon antibody
STJ95129Anti-PKC zeta antibody
STJ95123Anti-PKC gamma antibody
STJ95118Anti-PKC alpha antibody
MBS8243032-01Anti-PKC theta Antibody
MBS9240687-01Anti-PKC delta Antibody
CQA8551-01Anti-PKC eta Antibody
MBS9233573-01Anti-PKC-pan Antibody
PAab06486Anti-PKC iota antibody

Popular Products