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Recombinant Human Poly (RC) Binding Protein 1

CAT: 0793-32-4402-20Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0793-32-4402-20Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Source : Escherichia Coli. PCBP1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 186 amino acids (1-163) and having a molecular mass of 19.6 kDa.PCBP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. PCBP1 is a single-stranded nucleic acid binding protein that binds preferentially to oligo dC. PCBP1 plays a role in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. PCBP1 along with PCBP-2 and hnRNPK corresponds to the major cellular poly (rC) -binding protein. PCBP1 and PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promotes poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure.
Product Name Alternative
Poly (rC) binding protein 1||hnRNP-E1||hnRNP-X||HNRPE1||HNRPX||Alpha-CP1||Heterogeneous nuclear ribonucleoprotein E1||Nucleic acid-binding protein SUB2.3||PCBP1.
Purification
Greater than 85.0% as determined by SDS-PAGE.
Components
The PCBP1 solution (1mg/mL) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Storage Conditions
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Avoid multiple freeze-thaw cycles.
Amino Acids
MGSSHHHHHH SSGLVPRGSH MGSMDAGVTE SGLNVTLTIR LLMHGKEVGS IIGKKGESVK RIREESGARI NISEGNCPER IITLTGPTNA IFKAFAMIID KLEEDINSSM TNSTAASRPP VTLRLVVPAT QCGSLIGKGG CKIKEIREST GAQVQVAGDM LPNSTERAIT IAGVPQSVTE CVKQIC.

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