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Recombinant Human RAN Binding Protein 1

CAT: 0793-32-4598-25Size: 25 µgDry Ice: NoHazardous: No
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CAT#:0793-32-4598-25Size:25 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Source : E.coli. RANBP1 Human Recombinant produced in E. coli is a single polypeptide chain containing 225 amino acids (1-201) and having a molecular mass of 25.8kDa (molecular size on SDS-PAGE will appear higher) .RANBP1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. RANBP1 is a member of a family of proteins which bind Ran GTPase. Ran GTPase is vital for cell cycle control, mRNA processing, nuclear transport, mitotic spindle assembly, and postmitotic nuclear re-assembly and nuclear architecture maintenance. RANBP1 does not stimulate GTPase activity of RAN however RANBP1 does distinctly raise GTP hydrolysis by the RanGTPase-activating protein (RanGAP1) . Additionally, RANBP1 operates as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.
Product Name Alternative
RAN binding protein 1||HTF9A||ran-specific GTPase-activating protein||RanBP1.
Purification
Greater than 95% as determined by SDS-PAGE.
Components
The RANBP1 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 10% glycerol.
Storage Conditions
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.
Amino Acids
MGSSHHHHHH SSGLVPRGSH MGSHMAAAKD THEDHDTSTE NTDESNHDPQ FEPIVSLPEQ EIKTLEEDEE ELFKMRAKLF RFASENDLPE WKERGTGDVK LLKHKEKGAI RLLMRRDKTL KICANHYITP MMELKPNAGS DRAWVWNTHA DFADECPKPE LLAIRFLNAE NAQKFKTKFE ECRKEIEERE KKAGSGKNDH AEKVAEKLEA LSVKEETKED AEEKQ.

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