GPBB Recombinant Protein

CAT: 0793-32-2385-20Size: 20 µgDry Ice: NoHazardous: No
CAT#:0793-32-2385-20Size:20 µg
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Description
Source : Escherichia Coli. Glycogen Phosphorylase Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain. The Human GPBB mature chain: 2 - 843 aa; that is a total of 842 aa having a molecular mass of 96695.96 Dalton. The theoretical pI is 6.40.The GPBB is purified by proprietary chromatographic techniques. Glycogen phosphorylase is one of the phosphorylaseenzymes (EC2.4.1.1) . It breaks up glycogeninto glucosesubunits. Glycogenis left with one less glucosemolecule, and the free glucosemolecule is in the form of glucose-1-phosphate. In order to be used for metabolism, it must be converted to glucose-6-phosphateby the enzyme phosphoglucomutase.Glycogen phosphorylase can only act on linearchainsof glycogen (a 1-4 glycosidic linkage) . Its work will immediately come to a halt four residues away from a 1-6 branch (which are exceedingly common in glycogen) . In these situations, a debranching enzymeis necessary, which will straighten out the chain in that area. Additionally, an alpha 1-6 glucosidaseenzymeis required to break the remaining 1-6 residue that remains in the new linear chain. After all this is done, glycogen phosphorylase can continue.An insulinstimulated enzyme known as phosphoprotein phosphatase (PP-1) inactivates glycogen phosphorylase to prevent glycogen break up.GPBB - a sensitive marker for the AMI diagnosis within 4 hours after the onset of chest pain. It has also been shown that GPBB is increased in a considerable proportion of AMI patients within 2-3 hours from chest pain onset. GPBB is increased early in patients with unstable angina. GPBB can also be a sensitive marker for the detection of peri-operative myocardial ischaemia and infarction in patients undergoing coronary artery bypass grafting.
Product Name Alternative
Glycogen phosphorylase brain form||EC 2.4.1.1||GPBB||MGC9213||PYGB.
Purification
Greater than 85.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Components
Each mg of protein contains 50% glycerol.
Storage Conditions
GPBB although stable at 10°C for 7 days, should be stored below -18°C. Please prevent freeze-thaw cycles.

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