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SRANKL Recombinant Protein

CAT: 0793-32-1717-10Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0793-32-1717-10Size:10 µg
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Description
Source : Escherichia Coli. sRANKL Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 20 kDa. RANKL binds to tnfrsf11b/opg and to tnfrsf11a/rank. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive t-cell proliferation. May be an important regulator of interactions between t-cells and dendritic cells and may play a role in the regulation of the t-cell-dependent immune response. sRANKL may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
Product Name Alternative
Soluble Receptor Activator of NFkB Ligand||TNFSF11||TRANCE||TNF-related activation-induced cytokine||OPGL||ODF||Osteoclast differentiation factor||Tumor necrosis factor ligand superfamily member 11||Receptor activator of nuclear factor kappa B ligand
Purification
Greater than 90.0% as determined by analysis by SDS-PAGE.
Components
The protein was lyophilized from a concentrated (1mg/mL) solution containing 10mM Na2PO4, pH-8.0.
Storage Conditions
Lyophilized TNFSF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sRANKL should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.
Applications Notes
It is recommended to reconstitute the lyophilized sRANKL in sterile 18MΩ-cm H2O at a concentration of 100μg/ml, which can then be further diluted to other aqueous solutions. The activity is determined by a dose-dependent stimulation of IL-8 production in human PBMC and is typically less than 100ng/ml, corresponding to a specific activity of 10,000 Units/mg.
Amino Acids
EKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID.

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