Products for Research Use Only
RegisterLogin

IL35 Recombinant Protein

CAT: 0793-32-1521-10Size: 10 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0793-32-1521-10Size:10 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Source : HEK 293 cells. IL35 Human Recombinant produced in HEK 293 cells is comprised of a p35 subunit from IL-12 (Accession # p29459) and a EBI3 (Accession # q14213) subunit from IL-27. IL35 is a heterodimeric polypeptide chain containing 442 amino acids, with a predicted molecular weight of 49 kDa. From N terminus to C terminus, the molecule is comprised of a poly His tag, the EBI3 subunit, a G rich linker and the p35 subunit. Interleukin-35 is a glycosylated, disulfide-linked, heterodimeric protein comprised of the p35 subunit of IL-12 (IL-12 alpha) and the beta subunit of IL-27 (EBI3) . IL35 is expressed by regulatory T-cells (Tregs), macrophages and some trophoblast and dendritic cells. IL-35 is produced in response to inflammation and generally serves as an inflammation suppressor. Interleukin-35 curbs inflammation by applying multiple activities, including the stimulation of regulatory T-cells and the inhibition of Th17 cells.
Purification
Greater than 95.0% as determined by analysis by SDS-PAGE.
Components
Lyophilized from a concentrated solution containing 10mM Na2PO4 pH 7.5 and 30mM NaCl.
Storage Conditions
Lyophilized IL35 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IL35 should be stored at 40C between 2-7 days and for future use below -180C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.
Applications Notes
It is recommended to reconstitute the lyophilized IL35 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Amino Acids
EBI3 subunit:RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRPRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGKp35 subunit:RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS

Related Products

CatalogName
32-1536-25IL37 Recombinant Protein
32-1520-10IL34 Recombinant Protein
32-17867-100Recombinant Human IL31 Protein, hFc Tag
32-17867-50Recombinant Human IL31 Protein, hFc Tag
32-4513-10Recombinant Protein L
32-3516-20CFL2 Recombinant Protein
32-3507-20CENPP Recombinant Protein
32-3506-10CENPM Recombinant Protein
32-3505-20CENPH Recombinant Protein
32-3502-20CEND1 Recombinant Protein

Alternative Products

CatalogName
PX-P6112-100Human IL35 recombinant protein
AP93188Recombinant Human IL35 Protein, N-His
GTR19139475-01Recombinant Human IL35 Protein, C-Fc
QP12423-2ugRecombinant Human IL35 Protein, His, HEK 293-2ug
QP12423-100ugRecombinant Human IL35 Protein, His, HEK 293-100ug
QP12423-10ugRecombinant Human IL35 Protein, His, HEK 293-10ug
abx263181-01IL35 Protein
GTR18984940-01Human IL35 Protein
MBS2011667-01Recombinant Interleukin 35 (IL35)
ARO-P12951-01Recombinant Human IL35, N-His

Popular Products