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PE conjugated anti CD65 (Clone VIM8)

CAT: 0793-30-2766-100Size: 100 TestsDry Ice: NoHazardous: No
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CAT#:0793-30-2766-100Size:100 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD65 is a fucosylated carbohydrate antigen (ceramide-dodecasaccharide, type II fucoganglioside), which serves as a ligand for CD62E (E-selectin) . Its structure is Gal beta1-4 GlcNAc beta1-3 Gal beta1-4 GlcNAc (3-1 Fuc alpha) beta1-3 ceramide. Unlike CD65s, the CD65 antigen does not contain terminal sialic acid, the rest of their structure is identical. CD65 is expressed on granulocytes and monocytes and participates in cell adhesion. It has been reported as important for extravascular infiltration of acute monocytic leukemia cells. Specificity :The mouse monoclonal antibody VIM8 recognizes human CD65, an asialo-fucoganglioside expressed on the surface of peripheral blood granulocytes (highly) and monocytes (weakly) .
Product Name Alternative
Ceramide-dodecasaccharide, type II fucoganglioside
Reactivity
Human
Immunogen
THP-1 cell line
Target Antigen
CD65
Clonality
Monoclonal
Clone
VIM8
Applications
FACS
Purification
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Components
Concentration: 1 mg/mLStorage Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage Conditions
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications Notes
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Conjugate
PE

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